Research Resources - All

A variety of research support services are available to assist University of Pittsburgh investigators in advancing their research programs. You can find resources categorically by choosing one of options below or search the entire directory through the keyword search option.

To submit updates, or to request the addition of a resource, please contact Paul Wood at pgw@pitt.edu or (412) 648-9618. 

 

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Virus Production (Recombinant)

Magee Women's Research Institute (MWRI)
204 Craft Avenue; Pittsburgh, PA 15213
(412) 641-2415
http://www.mwrif.org/302

Viral Vector Production

The Genome Editing, Transgenic, and Virus Core (GETV) facility provides lentivirus, adenovirus and adeno-associated virus vector design assistance and production. The Core maintains an inventory of stock plasmids and viral vectors that are available for a nominal fee.

Lentiviral vectors are produced in 293FT cells using a four plasmid system and are replication defective. Lentiviral vectors prepared using this system can be used at BSL2.

Adeno-associated virus 2 (AAV2) is produced in 293 cells and all vectors are purified for distribution. Purity is confirmed by SDS-PAGE and densitometry. A certificate of analysis is available upon request. AAV vectors prepared in this way are suitable for work at biosafety level 1 (BSL1).

Adenoviral vectors are produced in 293AD cells and purified for distribution. Adenoviral vectors prepared using this system can be used at BSL2.

For more information and to place an order link to the order form.

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