Research Resources - Laboratory and Computing
This directory provides links or contact information for laboratory, computing, and data analysis resources that are available to University investigators.
To submit updates, or to request the addition of a resource, please contact Paul Wood at email@example.com or (412) 648-9618.
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Division of Laboratory Animal Resources (DLAR)
The Division of Laboratory Animal Resources (DLAR) provides education, training, veterinary and animal husbandry care, and administrative services to support the institution’s animal based research and teaching needs. The DLAR coordinates efforts to provide a humane, quality animal care program in compliance with legal and regulatory requirements. The programs and facilities are USDA registered, covered under an Assurance with the Office of Lab Animal Welfare (OLAW) of the PHS, and accredited by the Association for Assessment and Accreditation of Laboratory Animal Care International (AAALAC). The DLAR forms and guidance documents may be found. Additional animal care policies may be found on the Institutional Animal Care and Use Committee’s website.
Drug Discovery Institute (UPDDI)
Vision for the University of PIttsburgh Drug Discovery Institute: Optimizing the discovery and development of new molecular entities through collaborations between faculty, academic centers, corporations and government agencies, together with innovations focused on Quantitative Systems Pharmacology (QSP).
The UPDDI has three major classes of activities: a) integrated programs that apply QSP to metastatic breast cancer, Huntington’s Disease (as a first focus area to be extended to other neurodegenerative diseases) and liver diseases; b) projects based on ligand- or target-centric discovery that are driven by faculty collaborators/ RO1 grants covering many therapeutic areas; and c) translational technology development including companion diagnostics, computational biology/chemistry tools, analytics to identify and quantify heterogeneity in experimental and clinical samples and advanced human tissue models for discovery. The UPDDI is positioned in an outstanding environment to translate key scientific and clinical advances into novel therapeutics.
Epidemiology Data Center
The Epidemiology Data Center (EDC) provides a research environment in which complex health questions can be explored and answered using the combined tools of biology and statistics. The EDC establishes collaborations with clinical researchers to design, conduct, and analyze multi-center randomized clinical trials and epidemiologic studies. To contribute to the development of new knowledge, the EDC develops and refines data collection, data management, computing, and statistical methods, with the ultimate goal of advancing treatment and prevention of disease.
The EDC has collaborated in over 100 research studies sponsored by the National Institutes of Health and other agencies. At any given time the EDC coordinates data management and analysis activities for 20 to 30 research projects sponsored by federal agencies as well as by industry. The current studies represent a variety of scientific designs including clinical trials, registries, and case control studies.
Genome Editing, Transgenic, and Virus Core (GETV)
The Genome Editing, Transgenic and Virus core (GETV) is dedicated to shepherding projects, from vector design to the establishment and maintenance of valuable transgenic/knockout/knockin lines. The core assists investigators by developing state-of-the-art strategies to solve complex biological problems using transgenic, gene editing and viral vector approaches. Directed by Dr. Kyle Orwig and managed by Dr. Yi Sheng, the core provides technical expertise, advice and services for investigators in the University of Pittsburgh community and beyond. Please see the website or contact us for further information or to schedule a face to face meeting to design and implement your experimental plans.
- Assistance for generating recombinant plasmids;
- Design and implementation of CRISPR/Cas9 technology for in vivo and in vitro work;
- Mouse and rat transgenesis, including transgenic and knockout/knockin techniques using pronuclear injection, ES cell injection/chimera and CRISPR/Cas9-mediated genome editing;
- Lentivirus, adenovirus and adeno-associated virus vectors;
- Cryopreserve or re-derive of valuable transgenic/knockout or other rodent lines using sperm or embryos;
- Additional animal testing and procedures: teratoma and chimera testing to validate putative pluripotent stem cell lines;
- GETV core services are approved by the Institutional Animal Care and Use Committee (IACUC), the recombinant DNA committee, and the environmental health and safety office of the University of Pittsburgh.
Genomics Research Core
The Genomics Research Core is equipped with state-of-the-art instrumentation and provides a variety of standard as well as customized genomic analyses to University researchers. The services include Sanger DNA sequencing, Next Generation sequencing of DNA and RNA, RNA/DNA extraction, purification and QC services, gene expression microarrays, whole transcriptome human and mouse microarrays, miRNA microarrays, TaqMan real-time PCR and Nanostring digital expression profiling for mRNA and miRNA, candidate gene and whole genome SNP array genotyping and NanoString CNV analysis. New technology and capabilities are added frequently; for a complete description of services and pricing, please visit the website.
The laboratory staff and leadership are available to provide expert advice and support with experimental design, new protocol development, technical support, data analysis and interpretation and assistance with manuscript and grant preparation including budgeting related to Genomics Research Core Services.
Health Sciences Core Research Facilities
The Health Sciences Core Research Facilities (HSCRF) were established through the Office of the Senior Vice Chancellor, Health Sciences in recognition of the vital role that centralized research resources play in the academic research enterprise. The technology required to perform innovative biomedical research changes rapidly and centralized research resources provide access to expertise, technology applications, and state-of-the-art instruments that are otherwise unavailable to most investigators.
Our research resource facilities include scientists and technicians who provide expert advice for study design, standard and custom applications and assist the investigator to take their project aims from conception to data production. The scientific leadership is available to work with you to adapt and apply methods appropriate to your scientific goals and assure that the interpretation of the results is supported by the data.
- Biomedical Mass Spectrometry Center (Proteomics, quantitation & other)
- Genomics Analysis Core (Bioinformatics)
- Genomics Research Core (RNA, DNA, Nextgen Sequencing)
- Health Sciences Metabolomics and Lipidomics Core
- Health Sciences Sequencing Core at Children's Hospital of Pittsburgh (Illumina sequencing)
- Health Sciences Tissue Bank
- Hillman Live Cell Imaging Core (DNA damage, confocal microscopy)
- Peptide and Peptoid Synthesis Core
- Small Molecule Biomarker Core (Quantitative Mass Spectrometry analysis)
Health Sciences Sequencing Core at Children's Hospital of Pittsburgh
The Health Sciences Sequencing Core at Children’s Hospital of Pittsburgh (HSSCatCHP) offers Illumina next generation sequencing services to the University of Pittsburgh research community. The core was established by a cooperative agreement between the University of Pittsburgh Schools of Health Sciences and the Children’s Hospital of Pittsburgh of UPMC.
Our personnel are experts in next generation sequencing technologies and are committed to remaining abreast of advances that will impact the research effort of investigators. We encourage you to contact our core managers Debby Hollingshead and William Horne to arrange for a consultation. Our staff is available to share their experience and expertise to assist with development of projects of all sizes, standard or custom assay design, and other specialized application needs.
Health Sciences Tissue Bank (HSTB)
The HSTB provides University of Pittsburgh researchers with excess tissue materials obtained from clinical procedures performed at some of the University of Pittsburgh Medical Center (UPMC) hospitals. The main purposes of the HSTB are: (1) to provide a mechanism to simplify and streamline the process of research tissue accrual, storage and disbursement and (2) to provide efficient research pathology support services.
Services provided by HSTB include:
- Human tissue and biological specimen procurement services
- Honest broker services
- Research histology services
- Annotation of clinical data
- Tissue microarray (TMA) services
The HSTB contains a variety of solid tissues, cell aspirates, blood, peripheral blood mononuclear cells, as well as other clinical samples. Solid tissues are available from an assortment of tumors and from various non-neoplastic disease states from sources including the gastrointestinal tract, liver, and lung.
True normal tissues procured for approved projects through a working relationship with the Center for Organ Recovery and Education (CORE) are also available. The HSTB develops procurement strategies on an ongoing basis for projects requiring tissue types or disease states that are not currently being collected. This effort includes working directly with investigators as an interface with clinics, surgical programs and the HSTB Rapid Autopsy Program.
The HSTB has IRB approval for a variety of collections including a specific HSTB IRB to collect tissues that would have otherwise been discarded. The HSTB provides service based on the existing HSTB IRB protocol if appropriate; it can assist investigators with submission of their own IRB protocols, when necessary.
Luminex Core Laboratory
This facility uses the Laboratory Multianalyte Profiling (LabMAP™) system developed by Luminex Corporation. It is a flexible, easy-to-use protein analysis system that permits the simultaneous quantitative analysis of up to 100 different proteins, peptides, or DNA molecules in a single microtiter well. Multiplexing enables a comprehensive overview of complex biological systems and the inclusion of in-process controls using a minimum amount of sample. Potential applications include:
- functional genomics
- validation of drug leads
- secondary screening
- absorption and distribution
- high-throughput ELISA and Western blot assays.
The UPCI Luminex Core Laboratory can process and analyze samples using commercially available manufactured kits or unique Core developed kits. The UPCI Luminex Core Laboratory can also develop custom analyte Luminex assays using investigator-supplied antibodies and a protein standard.
The CBP/Pharmacology Machine Shop provides consultation, design and fabrication services to School of Medicine investigators who need specialized devices for experimental use and specialized clinical devices. The Shop personnel can diagnose and repair mechanical scientific machinery and equipment, and working in conjunction with the CBP/Pharmacology Electronics Shop, can also address the repair of electronically controlled scientific machinery and equipment. Services are fee based and an account is required at the time of request of service.