Research Resources - Laboratory and Computing
This directory provides links or contact information for laboratory, computing, and data analysis resources that are available to University investigators.
To submit updates, or to request the addition of a resource, please contact Paul Wood at firstname.lastname@example.org or (412) 648-9618.
For Pitt Biomedical Research Facilities' web site search.
View Resources by Category
Magee Womens Research Institute (MWRI) Core Services
For information about each of the MWRIF Core services please go to their web pages or email at the links below.
Flow Cytometry Research Core Web Link
Genome Editing, Transgenic & Virus (GETV) Core Web Link
Histology & Micro-Imaging Core Web Link
Obstetric Specimen Procurement Unit (OSPU)
Magee Obstetric Medical and Infant (MOMI) Database Web Link
Clinical Research and Translational Center (CTRC) Web Link
Peptide and Peptoid Synthesis Core
The Peptide and Peptoid Synthesis Core provides comprehensive services for synthesis, purification, and characterization of synthetic peptides and peptoids. State-of-the-art microwave technology enables the high quality production of long and otherwise difficult to synthesize sequences. The purity and integrity of each synthesis product is confirmed by HPLC and mass spectrometry.
Facility personnel are available for consultation with investigators regarding the design of synthesis peptides and peptoids, estimates of yield, and considerations of purity requirements. Peptides and peptoids can be produced at standard scales of 0.025 mmol (10-20mg), 0.05 mmol (20 -40 mg), 0.1 mmol (50-100mg), 0.2 mmol (100-200mg), and 0.5 mmol (300-500mg), with actual yields dependent on peptide length and content.
Peptides can be prepared with custom modifications including but not limited to acetylation, biotinylation, phosphorylation, cyclization, and fluorescent dyes. The facility has the capacity to produce certified peptides for use in phase I and II human clinical trials with appropriate production documentation for submission to the FDA and other regulatory agencies.
Pitt Gnotobiotic Facility
The Pitt Gnotobiotic facility is a fee-for-service core that allows for experiments requiring germ-free (without bacteria, fungi and exogenous viruses) and gnotobiotic (‘known microbiota’) mice. The facility allows for the use of commercially available strains of mice in addition to a limited selection of ‘knockout’ mice. Use of the facility is determined on a first come/first served basis, but the committee on gnotobiotics reserves the right to limit the size and scope of experiments to allow for multiple users. Assistance in gavaging or otherwise associating mice with microbiota is possible. Keywords: mouse microbiome animal core
The facility is planning to be operational by Fall 2018.
Pittsburgh Supercomputing Center
Pittsburgh Supercomputing Center provides university, government, and industrial researchers with access to several of the most powerful systems for high-performance computing, communications and data-handling available to scientists and engineers nationwide for unclassified research. PSC advances the state-of-the-art in high-performance computing, communications and informatics and offers a flexible environment for solving the largest and most challenging problems in computational science. As a leading partner in XSEDE, the National Science Foundation program of coordinated cyberinfrastructure for education and research, PSC works with its XSEDE partners to harness the full range of information technologies to enable discovery in U.S. science and engineering.
Rangos (Children’s Hospital of Pittsburgh) Core Facilities
For a complete listing of research resources available through the Children’s Hospital of Pittsburgh (CHP), please link to their Core Facilities web page.
- Cell Imaging Core I Laboratory
- Cell Imaging Core II Laboratory
- Flow Cytometry Core Laboratory
- Histology Core Laboratory
- Animal Imaging Core Laboratory
Rodent Behavior Analysis Core (RBAC)
The Rodent Behavior Analysis Core (RBAC) is a core facility at the University of Pittsburgh Schools of the Health Sciences. The purpose of the RBAC is to facilitate characterization of the behavioral phenotype of rat and mouse models studied by investigators throughout the University of Pittsburgh and its affiliated centers, institutes, and hospitals. To that end, the RBAC provides:
- advice on the choice and design of behavioral test batteries
- training in the application of behavioral protocols and associated data collection
- access to a wide range of state-of-the-art behavioral testing equipment and paradigms
- advice on basic data management, analysis, and interpretation
Additional Keywords: animal
Small Molecule Biomarker Core (SMBC)
The SMBC utilizes state-of-the-art instrumentation to provide quantitative analysis of specific analytes in clinical samples and method development for analytes where a method does not currently exist. The highly sensitive and specific techniques employed by the Core provide accurate quantitation for multiple biomarkers from the same sample, which maximizes the ability to link basic science with clinical outcomes.
Methods are currently available to analyze many compounds including: cyclooxygenase, CYP450 and lipoxygenase metabolites of arachidonic acid, progestins, estrogens, neurosteroids, and vitamin D metabolites. A more detailed overview, including current methods available from the core, is available at the web site.
Statistics Consulting Center
The Center for Statistics offers statistical consulting services, including design, data summarization, analysis, and interpretation of results. Researchers from various disciplines (within the arts and sciences, as well as clinical research and education) are welcome. Typically, only short-term consulting projects can be undertaken. The consulting is done by graduate students under the supervision of faculty members in the Department of Statistics.
Transgenics and Gene Targeting
The TGT (Transgenic and Gene Targeting) and Innovative Technologies Development (ITD) Cores in the Department of Immunology are a shared resource designed to produce genetically engineered mice for investigators of University of Pittsburgh. Our laboratory provides a broad, comprehensive range of services to investigators.
Keywords: Animal, genetic, vector, CRISPR/Cas9, rederivation, mouse, cryopreserve, knockin/knockout, chimera, chimeric
Unified Flow Core
The Unified Flow Core provides state-of-the-art flow cytometry and cell sorting services to the entire University of Pittsburgh research community as well as researchers at neighboring institutions. A skilled staff provides help with instrument setup, data analysis, and consultation for experiment design. Training is available to enable investigators and their staff to run the analytical cytometers themselves.
The Unified Flow Core has 3 LSR Fortessas (1 bio-contained), 2 LSR IIs, 3 FACSAria cell sorters (2 bio-contained) and 1 ImageStreamx Mark II