Research Resources - Laboratory and Computing

This directory provides links or contact information for laboratory, computing, and data analysis resources that are available to University investigators. 

To submit updates, or to request the addition of a resource, please contact Paul Wood at pgw@pitt.edu or (412) 648-9618.

 

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Virtual Handbook for Researchers with Animal Subjects

Website

The Division of Laboratory Animal Resources (DLAR) maintains this site to provide the research community and those whose research includes animals with information and important links that will assist them in establishing and maintaining robust research programs.

Virus Production (Recombinant)

Magee Women's Research Institute (MWRI)
204 Craft Avenue; Pittsburgh, PA 15213
(412) 641-2415
http://www.mwrif.org/302

Viral Vector Production

The Genome Editing, Transgenic, and Virus Core (GETV) facility provides lentivirus, adenovirus and adeno-associated virus vector design assistance and production. The Core maintains an inventory of stock plasmids and viral vectors that are available for a nominal fee.

Lentiviral vectors are produced in 293FT cells using a four plasmid system and are replication defective. Lentiviral vectors prepared using this system can be used at BSL2.

Adeno-associated virus 2 (AAV2) is produced in 293 cells and all vectors are purified for distribution. Purity is confirmed by SDS-PAGE and densitometry. A certificate of analysis is available upon request. AAV vectors prepared in this way are suitable for work at biosafety level 1 (BSL1).

Adenoviral vectors are produced in 293AD cells and purified for distribution. Adenoviral vectors prepared using this system can be used at BSL2.

For more information and to place an order link to the order form.

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