Research Resources - Laboratory and Computing

This directory provides links or contact information for laboratory, computing, and data analysis resources that are available to University investigators. 

To submit updates, or to request the addition of a resource, please contact Paul Wood at pgw@pitt.edu or (412) 648-9618.

 

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University of Pittsburgh Cancer Institute (UPCI)

https://upci.upmc.edu/facilities.cfm

For a complete listing of research resources available as part of the University of Pittsburgh Cancer Institute (UPCI), please link to the UPCI Shared Resources web page.

  • Animal Facility
  • Biobehavioral Oncology Facility
  • Biostatistics Facility
  • Cancer Bioinformatics Services
  • Cancer Genomics Facility
  • Cancer Pharmacokinetics and Pharmacodynamics Facility
  • Cancer Proteomics Facility (Mass Spectrometry, Luminex)
  • Cell and Tissue Imaging Facility
  • Chemical Biology Facility
  • Cytometry Facility (Analytical cytometry, Flow cytometry, Imaging cytometry, FACS )
  • Hematopoietic Stem Cell Laboratory
  • Immunologic Monitoring and Cellular Products Laboratory
  • In Vivo Imaging Facility
  • Investigational Drug Services
  • Tissue and Research Pathology Services

Virtual Handbook for Researchers with Animal Subjects

Website

The Division of Laboratory Animal Resources (DLAR) maintains this site to provide the research community and those whose research includes animals with information and important links that will assist them in establishing and maintaining robust research programs.

Virus Production (Recombinant)

Magee Women's Research Institute (MWRI)
204 Craft Avenue; Pittsburgh, PA 15213
(412) 641-2415
http://www.mwrif.org/302

Viral Vector Production

The Genome Editing, Transgenic, and Virus Core (GETV) facility provides lentivirus, adenovirus and adeno-associated virus vector design assistance and production. The Core maintains an inventory of stock plasmids and viral vectors that are available for a nominal fee.

Lentiviral vectors are produced in 293FT cells using a four plasmid system and are replication defective. Lentiviral vectors prepared using this system can be used at BSL2.

Adeno-associated virus 2 (AAV2) is produced in 293 cells and all vectors are purified for distribution. Purity is confirmed by SDS-PAGE and densitometry. A certificate of analysis is available upon request. AAV vectors prepared in this way are suitable for work at biosafety level 1 (BSL1).

Adenoviral vectors are produced in 293AD cells and purified for distribution. Adenoviral vectors prepared using this system can be used at BSL2.

For more information and to place an order link to the order form.

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