Research Resources - Laboratory and Computing
This directory provides links or contact information for laboratory, computing, and data analysis resources that are available to University investigators.
To submit updates, or to request the addition of a resource, please contact Paul Wood at firstname.lastname@example.org or (412) 648-9618.
For Pitt Biomedical Research Facilities' web site search.
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Cell Culture and Cytogenetics Facility (CCCF)
The Cell Culture and Cytogenetics Facility provides comprehensive cytogenetics services as well assisting with study design and manuscript preparation.
Services include preparation, laboratory processing and interpretation for:
- Cell culture including primary cell culture of human cells, and EBV transformation of human B-lymphocytes and cell line cryopreservation
- Molecular probes
- Classical and molecular cytogenetic analyses of human and other mammalian cells including Karyotyping
- Cytogenetic harvests
- FISH and molecular genetic procedures requisite for FISH, including polymerase chain reaction (PCR) amplification, fluorescence and light microscopy, darkroom for standard photomicrography and printing as well as digital imaging and printing.
- Cell line authentication
- Mycoplasma testing
These approaches can be used to characterize the karyotype of cells; map the location of new genes, virus, or transgene insertions; monitor established cell lines to rule out interspecies cell culture contamination or other changes during cell culture; ensure the fidelity of embryonic stem cells prior to production of knockout mice; determine the pattern of chromosomal gains and losses; and enumerate copy numbers of chromosomes or cancer-related genes.
Keywords: Animal, Rigor and Reproducibility
Division of Laboratory Animal Resources (DLAR)
The Division of Laboratory Animal Resources (DLAR) provides education, training, veterinary and animal husbandry care, and administrative services to support the institution’s animal based research and teaching needs. The DLAR coordinates efforts to provide a humane, quality animal care program in compliance with legal and regulatory requirements. The programs and facilities are USDA registered, covered under an Assurance with the Office of Lab Animal Welfare (OLAW) of the PHS, and accredited by the Association for Assessment and Accreditation of Laboratory Animal Care International (AAALAC). The DLAR forms and guidance documents may be found. Additional animal care policies may be found on the Institutional Animal Care and Use Committee’s website.
Genome Editing, Transgenic, and Virus Core (GETV)
The Genome Editing, Transgenic and Virus core (GETV) is dedicated to shepherding projects, from vector design to the establishment and maintenance of valuable transgenic/knockout/knockin lines. The core assists investigators by developing state-of-the-art strategies to solve complex biological problems using transgenic, gene editing and viral vector approaches. Directed by Dr. Kyle Orwig and managed by Dr. Yi Sheng, the core provides technical expertise, advice and services for investigators in the University of Pittsburgh community and beyond. Please see the website or contact us for further information or to schedule a face to face meeting to design and implement your experimental plans.
- Assistance for generating recombinant plasmids;
- Design and implementation of CRISPR/Cas9 technology for in vivo and in vitro work;
- Mouse and rat transgenesis, including transgenic and knockout/knockin techniques using pronuclear injection, ES cell injection/chimera and CRISPR/Cas9-mediated genome editing;
- Lentivirus, adenovirus and adeno-associated virus vectors;
- Cryopreserve or re-derive of valuable transgenic/knockout or other rodent lines using sperm or embryos;
- Additional animal testing and procedures: teratoma and chimera testing to validate putative pluripotent stem cell lines;
- GETV core services are approved by the Institutional Animal Care and Use Committee (IACUC), the recombinant DNA committee, and the environmental health and safety office of the University of Pittsburgh.
Pitt Gnotobiotic Facility
The Pitt Gnotobiotic facility is a fee-for-service core that allows for experiments requiring germ-free (without bacteria, fungi and exogenous viruses) and gnotobiotic (‘known microbiota’) mice. The facility allows for the use of commercially available strains of mice in addition to a limited selection of ‘knockout’ mice. Use of the facility is determined on a first come/first served basis, but the committee on gnotobiotics reserves the right to limit the size and scope of experiments to allow for multiple users. Assistance in gavaging or otherwise associating mice with microbiota is possible. Tim Hand is willing to discuss experiment design and specifics to help ensure project success.
Available strains: C57BL/6, Balb/c
Soon: Rag1-/- on C57Bl/6 background
Rodent Behavior Analysis Core (RBAC)
The Rodent Behavior Analysis Core (RBAC) is a core facility at the University of Pittsburgh Schools of the Health Sciences. The purpose of the RBAC is to facilitate characterization of the behavioral phenotype of rat and mouse models studied by investigators throughout the University of Pittsburgh and its affiliated centers, institutes, and hospitals. To that end, the RBAC provides:
- advice on the choice and design of behavioral test batteries
- training in the application of behavioral protocols and associated data collection
- access to a wide range of state-of-the-art behavioral testing equipment and paradigms
- advice on basic data management, analysis, and interpretation
Additional Keywords: animal
Transgenics and Gene Targeting
The TGT (Transgenic and Gene Targeting) and Innovative Technologies Development (ITD) Cores in the Department of Immunology are a shared resource designed to produce genetically engineered mice for investigators of University of Pittsburgh. Our laboratory provides a broad, comprehensive range of services to investigators.
Keywords: Animal, genetic, vector, CRISPR/Cas9, rederivation, mouse, cryopreserve, knockin/knockout, chimera, chimeric